Molecular Biology Papers
Association mapping and localization of molecular markers linked to resistance in sugarcane to brown rust
Last Updated: junio 11, 2025Sugarcane brown rust (Puccinia melanocephala) is a disease of major importance in many sugar producing regions of the world, causing significant yield losses and economic impacts. The most effective method for controlling this disease is the use of resistant commercial varieties.
CRISPR/Cas9 mediated homology directed repair enables precise modification in sugarcane ALS gene
Last Updated: junio 11, 2025We investigated CRISPR/Cas9 homology-directed repair (HDR)-mediated introduction of the W574L mutation aimed at obtaining tolerance to acetolactate synthase (ALS) inhibiting herbicides. Specific sgRNAs were designed and inserted into Cas9-expressing gene editing vectors.
Field-based prediction of sugarcane photosynthesis through environmental inputs
Last Updated: junio 11, 2025Sugarcane (Saccharum hybrids) is a highly productive C4 crop prevalent in tropical and subtropical areas. However, its photosynthetic efficiency is influenced by environmental factors such as light, moisture and temperature. Understanding these interactions is critical for optimizing yields and addressing climate-related challenges.
Field-based prediction of sugarcane photosynthesis through environmental inputs
Last Updated: junio 11, 2025Sugarcane (Saccharum hybrids) is a highly productive C4 crop prevalent in tropical and subtropical areas. However, its photosynthetic efficiency is influenced by environmental factors such as light, moisture and temperature. Understanding these interactions is critical for optimizing yields and addressing climate-related challenges.
Genetic understanding of drought tolerance in sugarcane
Last Updated: junio 11, 2025Drought tolerance is a desirable breeding characteristic for Colombian sugarcane cultivars, supporting the sustainable development goals of the country's agriculture.
Quantification of transgene copies in genetically modified borer-resistant sugarcane
Last Updated: junio 11, 2025The South African Sugarcane Research Institute is developing genetically modified (GM) sugarcane with enhanced resistance to Eldana saccharina by introducing Cry1Ab and Cry2Ab genes. Two genotypes, T10K and TN71, were selected as suitable candidates for transformation, representing South African sugarcane industry's rainfed and irrigated regions, respectively
Sugarcane lines expressing the sunflower transcription factor HAHB11 have improved tolerance to abiotic stresses
Last Updated: junio 11, 2025Climate change is impacting crop production worldwide, causing significant yield losses. Genetic transformation represents an essential biotechnological tool for mitigating abiotic stress in crops. In this study, we introduced the sunflower transcription factor Hahb11 gene into the elite sugarcane variety TUC 03-12 by the biolistic transformation method.
Sustainable sugarcane: developing varieties resilient to pests and climate change through genetic transformation and genome editing
Last Updated: junio 11, 2025Sugarcane is a crucial crop in Colombia, serving as a key source of sugar, bioethanol, and cogeneration of electricity, and is a pillar of the agricultural economy. However, it faces significant threats from pests such as Diatraea sp. and water stress.
The complex polyploid genome architecture of sugarcane: genome assembly and origin of cultivar R570
Last Updated: junio 11, 2025In 2024, we produced a polyploid genome assembly of the modern cultivar R570. This assembly was obtained using a combination of approaches and sequencing technologies and resulted in 67 assembled chromosomes. We found that many chromosome segments, representing ~50% of the genome, were nearly identical and thus collapsed in the assembly.
Using the Axiom SNP array for comparative mapping across four Australian sugarcane varieties
Last Updated: junio 11, 2025Comparative mapping in sugarcane has had limited application due to initially markers such as AFLPs being used to generate maps for the large genome with no sequence information attached.